Construction Projects
Batch Biofilm Growth Reactor
Building a Biofilm Model from a Confocal Microscope Stack
Building and Using a Biofilm Continuous Flow Stirred Reactor
Building and Using a Flow Cell Biofilm Reactor

Techniques & Protocols
Collecting Soil Biofilms by the Buried Slide Technique
Colorimetric Measurement of Biofilm Density
Drop Plate Method for Counting Biofilm Cells
Flow Through Gram Stain
Harvesting and Dispersing of Cells from Biofilms (Standard Method)
Harvesting and Dispersing of Cells from Biofilms (Alternative Method)
Henrici’s Microbial Capture Technique
Measurement of Biofilm Thickness
Model System for Growing a Standard Biofilm: Static Glass Coupon Reactor

Biofilm Application Exercises
Biofilms as Biobarriers
Buccal Epithelial Cells with Adherent Bacterial Cells Revealed by Negative Staining
Case Presentation: Bacterial Infection in a Cystic Fibrosis Patient
Dental Biofilms: Detection and Quantification of Plaque
Isolation of Biofilm Populations from Soil Crumbs by Flotation

Notes for Teachers
Additional information for high school teachers

Acknowledgements

This work has been supported in part by the National Science Foundation under Grant No. 0618744, and in part by the Waksman Foundation for Microbiology. Developed in collaboration with Dr. John Lennox, Penn State Altoona. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the author(s) and do not necessarily reflect the views of the National Science Foundation.

Return to top